Production of medium chain length polyhydroxyalkanoates by fed-batch culture of Pseudomonas oleovorans |
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Authors: | Beom Soo Kim |
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Institution: | (1) Department of Chemical Engineering, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea |
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Abstract: | Pseudomonas oleovorans was cultivated to produce medium chain length polyhydroxyalkanoates (MCL-PHAs) from octanoic acid and ammonium nitrate as carbon and nitrogen source, respectively, by a pH-stat fed-batch culture technique. The octanoate in the culture broth was maintained below 4 g l–1 by feeding the mixture of octanoic acid and ammonium nitrate when the culture pH rose above 7.1. The final cell concentrations of 63, 55 and 9.5 g l–1, PHA contents of 62, 75 and 67% of dry cell wt, and productivities of 1, 0.63 and 0.16 g l–1 h–1 were obtained when the C/N ratios in the feed were 10, 20 and 100 g octanoic acid g–1 ammonium nitrate, respectively. |
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Keywords: | fed-batch culture medium-chain-length polyhydroxyalkanoates PHA Pseudomonas oleovorans |
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