Nuclear and cytoplasmic receptors for 1,25-dihydroxycholecalciferol in intestinal mucosa |
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Authors: | P F Brumbaugh M R Haussler |
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Affiliation: | Department of Biochemistry, College of Medicine University of Arizona, Tucson, Arizona 85724 USA |
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Abstract: | The apparent hormonal form of cholecalciferol, 1,25-dihydroxycholecalciferol (1,25-(OH)2-CC), was incubated with intestinal mucosa homogenates and whole intestinal tissue, . After 40–70 min, 1,25-(OH)2-CC was specifically associated with the nuclear chromatin fraction. This sterol remains bound to the cytosol fraction at 0°C and a dramatic movement to the nuclear chromatin occurs at 37°C indicating that the subcellular localization of the sterol is temperature dependent. Isolated intestinal cytosol, previously incubated with 1,25-(OH)2-CC, is required for transportation of the hormone to the intestinal chromatin fraction; cytosol fractions from other tissues are ineffective mediators of this sterol migration. It is concluded that the intestinal cytosol contains a specific receptor that functions to transport 1,25-(OH)2-CC to the nucleus, its probable site of action. |
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