Reflection-mode TERS on Insulin Amyloid Fibrils with Top-Visual AFM Probes |
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Authors: | Manola Moretti Remo Proietti Zaccaria Emiliano Descrovi Gobind Das Marco Leoncini Carlo Liberale Francesco De Angelis Enzo Di Fabrizio |
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Institution: | 1. Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy 2. BIONEM Lab, University of Magna Graecia, Campus S. Venuta, Germaneto, viale Europa, 88100, Catanzaro, Italy
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Abstract: | Tip-enhanced Raman spectroscopy provides chemical information while raster scanning samples with topographical detail. The coupling of atomic force microscopy and Raman spectroscopy in top illumination optical setup is a powerful configuration to resolve nanometer structures while collecting reflection mode backscattered signal. Here, we theoretically calculate the field enhancement generated by TER spectroscopy with top illumination geometry and we apply the technique to the characterization of insulin amyloid fibrils. We experimentally confirm that this technique is able to enhance the Raman signal of the polypeptide chain by a factor of 105, thus revealing details down to few molecules resolution. |
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