Cutting edge: cyclooxygenase-2 activation suppresses Th1 polarization in response to Helicobacter pylori |
| |
Authors: | Meyer Frank Ramanujam Kalathur S Gobert Alain P James Stephen P Wilson Keith T |
| |
Institution: | Division of Gastroenterology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA. |
| |
Abstract: | Helicobacter pylori infection causes a Th1-driven mucosal immune response. Cyclooxygenase (COX)-2 is up-regulated in lamina propria mononuclear cells in H. pylori gastritis. Because COX-2 can modulate Th1/Th2 balance, we determined whether H. pylori activates COX-2 in human PBMCs, and the effect on cytokine and proliferative responses. There was significant up-regulation of COX-2 mRNA and PGE(2) release in response to H. pylori preparations. Addition of COX-2 inhibitors or an anti-PGE(2) Ab resulted in a marked increase in H. pylori-stimulated IL-12 and IFN-gamma production, and a decrease in IL-10 levels. Addition of PGE(2) or cAMP, the second messenger activated by PGE(2), had the opposite effect. Similarly, stimulated cell proliferation was increased by COX-2 inhibitors or anti-PGE(2) Ab, and was decreased by PGE(2). Our findings indicate that COX-2 has an immunosuppressive role in H. pylori gastritis, which may protect the mucosa from severe injury, but may also contribute to the persistence of the infection. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|