Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase |
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| Authors: | Precigou Sylvain Wieser Marco Pommares Patrick Goulas Philippe Duran Robert |
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| Affiliation: | Laboratoire d'Ecologie Moléculaire, IBEAS, EA 3525, Université de Pau et des Pays de l'Adour, avenue de l'Université, UFR Sciences et Techniques, BP 1155 F-64013, Pau cedex, France. |
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| Abstract: | Rhodococcus pyridinovorans MW3 was isolated from an arable land of manioc from the Congo for its ability to transform acrylonitrile to acrylamide. This strain contains a cobalt nitrile hydratase (NHase) showing high sequence homology with NHases so far described. The specific NHase activity was 97 U mg(-1) dry wt. NHase production by R. pyridinovorans MW3 was urea and Co-dependent. The NHase was active for acrylamide up to 60% (w/v) indicating its potential for acrylamide production. |
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| Keywords: | acrylamide acrylonitrile nitrile hydratase NHase Rhodococcus pyridinovorans |
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