Novel oligosaccharides with the sialyl-Lea structure in human milk |
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| Authors: | H Kitagawa H Nakada S Fukui I Funakoshi T Kawasaki I Yamashina S Tate F Inagaki |
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| Affiliation: | Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan. |
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| Abstract: | We have isolated four novel oligosaccharides with the sialyl-Lea structure from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. The results of structural analyses, i.e., 500-MHz 1H NMR spectroscopy, fast atom bombardment mass spectrometry, and binding to specific anticarbohydrate antibodies, are consistent with the following structures. (formula; see text) |
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