| 兔GHR基因单核苷酸多态性及其与屠宰性状的相关性 |
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| 引用本文： | 邓小松,万洁,陈仕毅,王彦,赖松家,蒋美山,徐敏. 兔GHR基因单核苷酸多态性及其与屠宰性状的相关性[J]. 遗传, 2008, 30(11): 1427-1432. DOI: 10.3724/SP.J.1005.2008.01427 |
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| 作者姓名： | 邓小松 万洁 陈仕毅 王彦 赖松家 蒋美山 徐敏 |
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| 作者单位： | 四川农业大学动物科技学院, 雅安 625014 |
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| 基金项目： | 公益性行业(农业)科研专项 |
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| 摘 要： | 摘要: 采用PCR-SSCP技术和DNA测序的方法, 对比利时兔、天府黑兔、齐卡巨型兔、哈尔滨白兔以及加利福尼亚兔5个肉兔品种的生长激素受体(Growth hormone receptor, GHR)基因进行单核苷酸多态性分析。结果发现了2个单核苷酸多态位点, 分别位于外显子10的705(T→C)和810 (C→T)。通过最小二乘分析SNPs及其与屠宰性状的关系发现, 基因型AA和MM所对应的活重、全净膛、屠宰率最小二乘均值都显著低于基因型BB和NN(P<0.05)。但各基因型饲料转化率最小二乘均值差异不显著(P>0.05)。由此可以初步推断, GHR基因是影响兔体重和屠宰率等屠宰性状的主要候选基因。
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| 关 键 词： | 屠宰性状 PCR-SSCP 生长激素受体基因 兔 |
| 收稿时间： | 2008-02-22 |
| 修稿时间： | 2008-05-05 |
The correlations between polymorphism of growth horm- one re-ceptor gene and butcher traits in rabbit |
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| DENG Xiao-Song,WAN Jie,CHEN Shi-Yi,WANG Yan,LAI Song-Jia,JIANG Mei-Shan,XU Min. The correlations between polymorphism of growth horm- one re-ceptor gene and butcher traits in rabbit[J]. Hereditas, 2008, 30(11): 1427-1432. DOI: 10.3724/SP.J.1005.2008.01427 |
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| Authors: | DENG Xiao-Song WAN Jie CHEN Shi-Yi WANG Yan LAI Song-Jia JIANG Mei-Shan XU Min |
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| Affiliation: | Animal Science and Technological College, Sichuan Agricultural University, Ya’an 625014, China |
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| Abstract: | Abstract: Five rabbit populations (Belgian hare, Tianfu black rabbit, Great line of Zika rabbit, Harbin white rabbit, and Cali-fornia rabbit) were used to analyze the polymorphism of growth hormone receptor (GHR) gene by PCR-SSCP. Results indi-cated that there were two mutation sites (C705T and C810T) in the 5 populations. The least square analyses showed that the live weight, visceraste weight, and slaughter percentage of AA and MM genotypes were significantly lower than BB and NN genotypes (P<0.05). In contrast, the GHR polymorphism had no significant difference for least squares means of feed trans-formation efficiency (P>0.05). It suggested that GHR gene may be a candidate gene responsible for butcher trait in rabbit. |
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| Keywords: | PCR-SSCP |
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