| 稀土离子(La~(3+)、Nd~(3+)、Ho~(3+)、Yb~(3+))对人红细胞膜Na~+/K~+ ATPase作用的初步研究 |
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| 引用本文： | 张金红,魏继中,吳隽平,张遂坡,徐友涵.稀土离子(La~(3+)、Nd~(3+)、Ho~(3+)、Yb~(3+))对人红细胞膜Na~+/K~+ ATPase作用的初步研究[J].中国生物化学与分子生物学报,1993,9(3):274-277. |
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| 作者姓名： | 张金红 魏继中 吳隽平 张遂坡 徐友涵 |
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| 作者单位： | 南开大学分子生物学研究所，南开大学化学系，南开大学分子生物学研究所，南开大学分子生物学研究所，南开大学分子生物学研究所 天津 300071，天津 300071，天津 300071，天津 300071，天津 300071 |
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| 摘 要： | 本文以低渗法从新鲜健康人红细胞中制得膜Na~+/K~+ ATPase,用于研究四种鑭系稀土离子(La~(3+)、Nd~(3+)、Ho~(3+)、Yb~(3+))对该酶的影响。结果表明,四种鑭系离子都对红细胞膜Na~+/K~+ ATPase的活性有抑制作用。当反应体系中的稀土离子浓度达50μmol/L时,Na~+/K~+ ATPase的活性所剩不多;低于50μmol/L鑭系离子时,以Nd~(3+)的抑制作用最强;金属离子螯合剂能阻止稀土离子对Na~+/K~+ ATPase的抑制作用。此项研究为稀土的生物效应的理论提供了一些新的证据。
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| 关 键 词： | Na~+/K~+ATPase Ln~(3+) 抑制作用 |
| 收稿时间： | 1993-06-20 |
The Effect of Some Rare Earth Ion Ln~(3+) on the Activity of Na~+/K~+ ATPase in Human Erythrocyte Membrane |
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| Zhang,Jin-hong Wei,Ji-zhong.The Effect of Some Rare Earth Ion Ln~(3+) on the Activity of Na~+/K~+ ATPase in Human Erythrocyte Membrane[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(3):274-277. |
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| Authors: | Zhang Jin-hong Wei Ji-zhong |
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| Institution: | (**) Wu, Jun-ping Zhang, Sui-po Xu, You-ban (Institute for Molecular Biology, Department of Chemistry~(**), Nankai University, Tianjin 300071 |
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| Abstract: | Erythrocyte membrane Na~+/K~+ATPase from healthy human blood was prepared by the method of Hypo-Osmosis. The effect of some Ln~(3+) (La~(3+), Nd~(3+), Ho~(3+), Yb~(3+)) on the activity of Na~+/K~+ATPase was studied. Experimental results show that Ln~(3+) can inhibit Na~+/K~+ ATPase in human erythrocyte membrane. When the concentration of Ln~(3+) was 50μmol/L, the Na~+/K~+ ATPase activity was almost inhibited completely. At concentration below 50μmol/L of Ln~(3+), the inhibitory effect of Nd~(3+) is much stronger than the other three. EDTA can prevent the inhibitory effect of Ln~(3+) on Na~+/K~+ ATPase. |
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| Keywords: | Na~+/K~+ATPase Ln~(3+) Inhibition |
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