Crystalline arrays of ribosomes in isolated pea chloroplasts |
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| Authors: | UTA M. PRAEKELT ARTHUR J. ROWE HARRY SMITH |
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| Affiliation: | Departments of Botany, University of Leicester, Leicester, U. K.;Departments of Biochemistry, University of Leicester, Leicester, U. K. |
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| Abstract: | Abstract Transmission electron microscopy of chloroplasts isolated by osmotic lysis of pea leaf protoplasts has revealed crystalline arrays of ribosomal particles associated with the thylakoid membranes. Optical diffraction techniques have established the crystallinity of the arrays and an image-enhancement technique has given an indication of ribosomal macrostructure. A model of crystal-packing is presented. This apparently artefactual induction of ribosome crystals should provide a valuable approach towards the elucidation of the details of the structure of chloroplast ribosomes. |
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| Keywords: | Pisum sativum pea chloroplasts ribosomes crystals ribosome structure |
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