Selection of affinity ligands for protein purification from proteolytic digests |
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| Authors: | Yuhui Yang Howard A. Chase Guoquan Liu |
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| Affiliation: | (1) Institute of Chemistry, Chinese Academy of Sciences, Beijing, China;(2) Department of Chemical Engineerng, University of Cambridge, Pembroke Street, Cambridge, UK |
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| Abstract: | A simple method for the selection of affinity ligands from proteolytic digests by affinity chromatography is proposed. A small proportion of the peptides in the trypsin digest of bovine serum albumin (BSA) or the pepsin digest of cytochrome are retarded on insulin-immobilised or HSA (human serum albumin)-immobilised affinity columns, respectively. The peptides in these selected fractions can be immobilised onto solid phases and used in affinity chromatography procedures for the purification of insulin or HSA. © Rapid Science Ltd. 1998 |
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