Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection ofCampylobacter jejuni antibodies,and comparison with a complement fixation test (CFT) |
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| Authors: | J. Oosterom C. H. den Uyl J. R. J. Bänffer S. Lauwers J. Huismans A. E. Busschbach F. G. J. Poelma R. Bellemans |
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| Affiliation: | (1) Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Hygiene, P.O. Box 1, 3720 BA Bilthoven, The Netherlands;(2) Laboratory of Food Microbiology and -Hygiene, Department of Food Science, Agricultural University, de Dreyen 12, 6703 BC Wageningen, The Netherlands;(3) Laboratory for Epidemiological Bacteriology, Municipal Public Health Service, P.O. Box 333, 3000 AH Rotterdam, The Netherlands;(4) Infectious Disease Unit, University Hospital, Free University of Brussels, Laarbeeklaan 101, 1090 Brussels, Belgium;(5) Department of Infectious Diseases and Hygiene, Municipal Public Health Service, P.O. Box 70032, 3000 LP Rotterdam, The Netherlands |
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| Abstract: | An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of total anti-Campylobacter immunoglobulins in human sera. In this assay disintegratedCampylobacter bacteria were used as the antigen. Absorption tests including other possibly enteropathogenic bacterial species showed that the ELISA system displayed a high immunological specificity forCampylobacter. Using this ELISA it was found that in about 80% ofCampylobacter patients theseCampylobacter antibodies are produced to almost maximal levels within 8 days after onset of disease, and that they may persist for at least 4 months. Indeed,Campylobacter antibodies were demonstrated at low levels in a large number of control sera. However, accepting an antibody titre of 1: 640 as indicative ofCampylobacter infection, the statistical sensitivity of the ELISA system was 77% and the specificity 95%. In an epidemiological survey a high association was demonstrated between the severity ofCampylobacter-related symptoms and antibody titre values. Assessment ofCampylobacter antibody titres by means of this ELISA and by a complement fixation test in 92 sera from index patients and contacts with and without symptoms showed a high association of results. |
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