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Reaction of substituted phenols with thermostable laccase bound to Bacillus subtilis spores
Authors:Jun Hirose  Masako Nasu  Haruhiko Yokoi
Institution:(1) Department of Applied Chemistry, Faculty of Engineering, Miyazaki University, Miyazaki, 889-2192, Japan
Abstract:A strain of B. subtilis produced 1.8 times more laccase on sporulation medium than on non-sporulation medium. Spores oxidized mono- and di-methoxyphenols (0.1 mM) at 50 °C. The half-life of laccase bound to spores was about 2 d and the substrate was repeatedly removed by spores recovered from the reaction mixture.
Keywords:Bacillus subtilis  4-chloroguaiacol  laccase  spore
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