Reaction of substituted phenols with thermostable laccase bound to Bacillus subtilis spores |
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Authors: | Jun Hirose Masako Nasu Haruhiko Yokoi |
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Institution: | (1) Department of Applied Chemistry, Faculty of Engineering, Miyazaki University, Miyazaki, 889-2192, Japan |
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Abstract: | A strain of B. subtilis produced 1.8 times more laccase on sporulation medium than on non-sporulation medium. Spores oxidized mono- and di-methoxyphenols (0.1 mM) at 50 °C. The half-life of laccase bound to spores was about 2 d and the substrate was repeatedly removed by spores recovered from the reaction mixture. |
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Keywords: | Bacillus subtilis 4-chloroguaiacol laccase spore |
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