Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors |
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Authors: | Venil N. Sumantran Anthony J. Tranguch Prasanta Datta |
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Affiliation: | Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606. |
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Abstract: | The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting experiments showed increased synthesis of the dehydratase protein in the presence of gyrase inhibitors; addition of rifampicin and chloramphenicol to cells actively synthesizing enzyme preventing new enzyme production. Increased expression of dehydratase by gyrase inhibitors was accompanied by relaxation of supercoiled DNA. |
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Keywords: | Vibrio costicola Moderately halophilic eubacteria DNA homology |
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