Cellular adhesion to collagen |
| |
Authors: | Thomas F. Linsenmayer Eileen Gibney Bryan P. Toole Jerome Gross |
| |
Affiliation: | The Developmental Biology Laboratory, Departments of Medicine and Anatomy, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA |
| |
Abstract: | BALB/3T3 cells were released from tissue culture plates with EGTA, and their rates of attachment to collagen gels polymerized on Millipore filters; were measured. Cell attachment in serum-free medium was 20–50% of that which occurred in medium containing 10% fetal calf serum (FCS). Cell attachment to gels pretreated with FCS and assayed in serum-free medium was identical with that of gels in FCS-containing medium. Thus, it seems there are two separate mechanisms of attachment to collagen; one involving direct attachment and a second mediated by a serum component(s) which binds to collagen. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|