Anion and cation permeability of a large conductance anion channel in the T84 human colonic cell line |
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Authors: | Luis Vaca Diana L Kunze |
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Institution: | (1) Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 77030 Houston, Texas |
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Abstract: | Summary A large conductance multi-state channel was identified and characterized in single channel recordings from cell-attached and excised patches of the human colonic tumor cell line, T84. The channel activity was dependent on the presence of both permeable cations and anions. In Na+-free symmetrical Cl– solutions or Cl–-free symmetrical Na+ solutions the channel was inactive. Addition of 5mm NaCl (Nal or KCl) induced channel activity. The selectivity sequence obtained from the shift in reversal potential was I–(1.9) > Cl–(1) > Na+(0.5) > K+(0.3). SO
4
2–
, SCN– (thiocyanate) and NMDG+ were impermeant. Multiple subconductance states were identified at all voltages explored (±90 mV). The minimum conductance encountered in symmetrical 100mm NaCl was a 15 pS substate, the maximum, 210 pS. The channel appeared to be composed of multiples of the 15 pS subunits which were reversibly blocked by the loop diuretic bumetanide (5
m).The authors wish to thank Morris Priddy and Charley Roberson for excellent technical assistance and Linda Pai and Steve Valder for participation in the early experiments. This study was supported by UPSH R01-DK39617 to A. Beaudet. L.V. was supported by a one-year fellowship from the Cystic Fibrosis Foundation. |
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Keywords: | patch clamp subconductance states chloride channel anion-cation permeability bumetanide |
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