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Isolation and characterization of a heteroglycan from the fruits of Astraeus hygrometricus
Authors:Maiti Debabrata  Chandra Krishnendu  Mondal Subhas  Ojha Arnab K  Das Debsankar  Roy Sadhan K  Ghosh Kaushik  Chakraborty Indranil  Islam Syed S
Institution:Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Abstract:A water-soluble polysaccharide was isolated from an alkaline extract of the fruits of the ectomycorrhizal fungus, Astraeus hygrometricus. It was found to contain D-mannose, D-glucose, and L-fucose in a molar ratio of 1:2:1. On the basis of total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR studies (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structure of the repeating unit of the polysaccharide was established as Formula: see text]
Keywords:Astraeus hygrometricus  Ectomycorrhiza  Polysaccharide  Structure  NMR spectroscopy
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