Cytokine gene expression in atopics: effect of IL-4 on IL-1 beta and IL-6 mRNA levels |
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| Authors: | D Gauchat J F Gauchat F Bettens A L de Weck B M Stadler |
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| Affiliation: | Institute for Clinical Immunology, University of Bern, Inselspital, Switzerland. |
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| Abstract: | Mononuclear cells from atopic blood donors showed increased IL-3 steady state mRNA levels. This finding complemented our earlier observations that cells from atopics also showed increased IL-4 but decreased IFN-gamma, IL-1 beta and IL-6 mRNA levels. Therefore, we investigated the effect of human recombinant IL-4 on cytokines mRNA levels in mononuclear cells from normals and atopics. In the presence of IL-4 steady state levels of IL-1 beta and IL-6 mRNA were decreased even if cells were co-stimulated with polyclonal activators such as PMA, PWM or PHA. No influence of IL-4 on granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 or IFN-gamma mRNA levels was observed with the exception of a decreased IFN-gamma mRNA level in PWM stimulated cells. |
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