Synchronization of cell division in root tips of seven major cereal species for high yields of metaphase chromosomes for flow-cytometric analysis and sorting |
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| Authors: | H F Kaeppler S M Kaeppler J H Lee K Arumuganathan |
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| Institution: | (1) Department of Agronomy, University of Wisconsin, 53706 Madison, WI, USA;(2) Center for Biotechnology, University of Nebraska, 68588-0665 Lincoln, NE, USA |
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| Abstract: | Plant tissues that yield large numbers of mitotic cells are useful for cytogenetic analyses and prerequisite to flow-cytometric
analysis and sorting of plant chromosomes. Synchronization of cell division in samples from which chromosomes are to be isolated
is necessary to ensure high yields of chromosomes. A method developed for synchronizing cell division in root tips ofVicia faba (Dolezel et al., 1992; Lucretti et al., 1993) has been modified, and parameters are presented for the effective synchronization
of cell division in root tips ofAvena sativa (oat),Hordeum vulgare (barley),Oryza sativa (rice),Secale cereale (rye),Sorghum bicolor (sorghum),Triticum aestivum (wheat), andZea mays (maize). Optimum parameters for metaphase arrest and collection of chromosomes from synchronized samples are also presented.
Using these parameters, the yield and quality of chromosome preparations are appropriate for flow-cytometric characterization
and sorting. |
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| Keywords: | cell division flow karyotype hydroxyurea plant chromosome sorting synchronization |
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