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| 骨髓基质干细胞诱导分化为神经元过程中miR-124和miR-128的表达 | |
| 引用本文： | 王晶晶,王春芳,党雯,李鹏飞,蔚洪恩.骨髓基质干细胞诱导分化为神经元过程中miR-124和miR-128的表达[J].中国组织化学与细胞化学杂志,2010,19(3):221-224. |
| 作者姓名： | 王晶晶 王春芳 党雯 李鹏飞 蔚洪恩 |
| 作者单位： | 1. 山西医科大学生物技术教研室,太原,030001 2. 山西医科大学附属第二医院药剂科,太原,030001 3. 山西医科大学生物技术教研室,太原,030001;第二军医大学解剖教研室,上海,200433 |
| 摘 要： | 目的探讨骨髓基质干细胞诱导分化为神经元过程中miR-124和miR-128的表达变化及作用。方法采用全骨髓培养法体外分离培养获得骨髓基质干细胞,取传代培养至第3代的骨髓基质干细胞,在神经干细胞培养液及细胞因子等条件下诱导其分化为神经元,倒置显微镜下观察其形态变化,应用ABI公司的TaqManMicroRNAAssaysreal-timePCR技术,检测miR-124和miR-128在诱导分化过程中的表达。结果 miR-124分化后神经元的表达是未分化BMSCs的0.051倍(P0.05);miR-128分化后神经元的表达是未分化BMSCs的0.070倍(P0.05)。结论 miR-124和miR-128在骨髓基质干细胞诱导分化为神经元过程中可能起重要作用。 |
| 关 键 词： | 骨髓基质干细胞 分化 神经元 miR-124 miR-128 |
EXPRESSIONS OF miR-124 AND miR-128 IN THE PROCESS OF BONE MARROW STROMAL CELLS DIFFERENTIATING INTO NEURAL CELLS |
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| Wang Jingjing,Wang Chunfang,Dang Wen,Li Pengfei,Wei Hongen.EXPRESSIONS OF miR-124 AND miR-128 IN THE PROCESS OF BONE MARROW STROMAL CELLS DIFFERENTIATING INTO NEURAL CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2010,19(3):221-224. | |
| Authors: | Wang Jingjing Wang Chunfang Dang Wen Li Pengfei Wei Hongen |
| Institution: | 1Department of Biotechnology, Shanxi Medical University, Taiyuan 030001;2Department of Pharmacy, The Second Affiliated Hospital, Shanxi Medical University, Taiyuan 030001;3Department of Anatomy, The Second Military Medical University, Shanghai 200433, China) |
| Abstract: | Objective To detect the changes and effects of miR-124 and miR-128 in the process of bone marrow stromal cells （BMSCs） differentiating into neural cells.Methods BMSCs were isolated and cultured in vitro. Then BMSCs of passage 3 were induced to differentiate into neural cells. The morphologic features and growth status of BMSCs and differentiated BMSCs were observed by inverted microscopy. ABI＇s TaqMan MicroRNA Assays real-time PCR was used to measure the expression of miR-124 and miR-128 in the BMSCs before and after induction.Results The level of miR-124 in differentiated BMSCs was 0.051-fold of that in BMSCs （P0.05）,and the level of miR-128 in differentiated BMSCs was 0.070-fold of that in BMSCs （P0.05）.Conclusion Both miR-124 and miR-128 may play an important role in the process of bone marrow stromal cells differentiating into neurons. |
| Keywords: | miR-124 miR-128 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |