Fatty acids in callus cultures: Influence of growth factors on fatty acid composition of total lipids in callus cells |
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| Authors: | Banibrata Pandey V.N. Gadgil |
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| Affiliation: | Tissue Culture Laboratory, Bose Institute, 93/1, A.P.C. Road, Calcutta-700009, India |
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| Abstract: | Callus tissues were derived from cotyledon segments of Cucumis melo and Cucumis sativus. Four combinations of growth factors, i.e., naphthalene acetic acid (NAA) plus coconut water (CW); NAA plus kinetin; NAA plus 6-benzylaminopurine (BAP) and indole 3-butyric acid plus BAP, were incorporated in the medium for callus initiation as well as for growth of excised callus in culture for six passages. The proportion of total saturated to unsaturated acids and the ratio of linoleic to linolenic acid was influenced by the change in the type of auxin and cytokinin in the combinations used. A many fold increase of myristic acid was recorded for the indole 3-butyric acid plus BAP combination. |
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| Keywords: | Cucurbitaceae cotyledon callus agar culture fatty acids growth factors α-naphthalene acetic acid kinetin 6-benzyl amino purine indole 3-butyric acid coconut water. BAP, 6-benzylaminopurine CW, coconut water IBA, indole 3-butyric acids Kn, kenitin NAA, napthalene acetic acid. |
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