Pectic polysaccharides of cabbage (Brassica oleracea) |
| |
Authors: | Barry J.H. Stevens Robert R. Selvendran |
| |
Affiliation: | ARC Food Research Institute, Colney Lane, Norwich, NR4 7UA, U.K. |
| |
Abstract: | Pectic substances extracted from cabbage cell walls with water, at 80°, and (NH4)2C2O4, at 80°, accounted for 45%(w/w) of the purified cell wall material. Only a small amount of neutral arabinan was isolated. Partial acid hydrolysis and methylation analysis revealed that the major pectic polysaccharide had a rhamnogalacturonan backbone to which a highly branched arabinan was linked, at C-4 of the rhamnose units, mainly through short chains of (1→4)-linked galactopyranose residues. The bulk of the soluble pectic substances had only small amounts of proteins associated with them. After further extraction of the depectinated material with 1M and 4M KOH, to remove the hemicelluloses, the cellulose residue was found to contain a pectic polysaccharide which was solubilized by treatment with cellulase. The general structural features of the pectic polymers are discussed in the light of these results. |
| |
Keywords: | Cruciferae cabbage cell wall material pectic substances polysaccharides arabinan oligosaccharides methylation analysis |
本文献已被 ScienceDirect 等数据库收录! |
|