Ovarian Development and Vitellin of the Mushroom Fly,Lycoriella mali (Diptera: Sciaridae) |
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| Institution: | 1. University of Catania, Department of Agriculture, Food and Environment, Catania, Italy;2. Université Côte D’Azur, INRA (French National Institute for Agricultural Research), CNRS, UMR ISA, 06000, Nice, France;1. Department of Computer Science and Artificial Intelligence, Wenzhou University, Wenzhou, 325035, China;2. School of Surveying and Geospatial Engineering, College of Engineering, University of Tehran, Tehran, Iran;3. Department of Computer Science, School of Computing, National University of Singapore, Singapore, Singapore;4. Shanghai Lixin University of Accounting and Finance, Shanghai, 201209, China;5. Department of Information Technology, Wenzhou Polytechnic, Wenzhou, 325035, China;1. MOE Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou 310058, China;2. Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215006, China;3. State Key Laboratory for Diagnostic and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Hangzhou 310000, China;4. Breast Disease Center, First Affiliated Hospital of Medicine College of Zhejiang University, Hangzhou 310006, China;5. Department of Cell and Chemical Biology, Oncode Institute, Leiden University Medical Center, 2300 RC Leiden, the Netherlands;6. Department of Surgery, School of Medicine, UC Davis, Davis, CA 95817, USA;7. State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Fudan University, 200433 Shanghai, China;8. Department of Pharmacology and Moores Cancer Center, University of California, San Diego, La Jolla, CA 94158, USA |
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| Abstract: | Ovarian Development and Vitellin of the Mushroom Fly, Lycoriella mali, were characterized. L. mali has a pair of ovaries, composed of 50–60 ovarioles, respectively. Ovarian development began at 1 day of pupation, and continued to become mature at 2 days after adult emergence. The vitellogenin (Vg) and vitellin (Vn) of L. mali were identified by native- and SDS-PAGE analyses. The Vn is composed of two subunits with a large subunit, L-Vn (190 kDa), and a small subunit, S-Vn (63 kDa). The Vg and Vn detected in the hemolymph and ovary, respectively, have the two equivalent subunits (190, 63 kDa), respectively. These two subunits of Vn gradually decreased in content during embryogenesis. We confirmed the presence of the Vg and Vn which were first detected in the 3 day-old female pupae by SDS-PAGE and Western blot analyses. It can be assumed that the Vn is synthesized as a precursor (Vg) in the fat body at 3 days after pupation and released into hemolymph. Then, the Vg is subsequently absorbed into the ovary at the same time. Twelve amino acid residues after the signal peptide at the N-terminus of L. mali S-Vn were sequenced and showed 70% homology to those of Anthonomus grandis vitellegenin. |
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