Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells |
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| Authors: | Zhi-bao Liu Jing-hua Zhang Jing-hua Gao Jian Shi |
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| Institution: | 1.Oncology Department, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei;2.Oncology Department, Cangzhou Central Hospital, Cangzhou, Hebei, China |
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| Abstract: | The aim of this study was to explore the effects of the lncRNA ENST00000623984 on colorectal cancer. In this study, the expression levels of ENST000000623984 were first examined in tumor tissue and adjacent normal tissue from 40 patients with colorectal cancer and LoVo cells using quantitative real-time PCR. By siRNA transfection, ENST00000623984 expression was knocked down. Using flow cytometry, cell cycle progression and cell viability were examined in basal and knockdown LoVo cells. The CCK-8 assay was used to assess the cell proliferation rate, and the Transwell assay was used to determine the migration and invasion abilities. The ENST000000623984 expression level was increased in colorectal cancer. Knockdown of ENST000000623984 reduced cell viability, proliferation rate, cell migration and invasion. These results suggested that lncRNA ENST000000623984 may be involved in colorectal cancer development.Key words: LncRNA, ENST00000623984, colorectal cancer, expression, PCR |
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