Purification of glutathione reductase from gerbil liver in two steps |
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| Authors: | N Le Trang K K Bhargava A Cerami |
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| Institution: | Laboratory of Medical Biochemistry, The Rockefeller University, 1230 York Avenue, New York, New York 10021 USA |
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| Abstract: | A new method for the isolation of glutathione reductase which successively utilizes chromatography on 2'-5'-ADP-Sepharose 4B and DEAE-Sepharose CL 6B, is described. With these two steps, it was possible to purify to homogeneity the glutathione reductase from gerbil liver. Some molecular properties of the purified enzyme are reported. |
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| Keywords: | glutathione reductase affinity chromatography ion-exchange chromatography |
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