Molecular and <Emphasis Type="Italic">in vitro</Emphasis> characterization of field isolates of bovine herpesvirus-1 |
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Authors: | Julian Ruiz-Saenz Jairo Jaime Gloria Ramirez Victor Vera |
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Institution: | 1.Faculty of Veterinary Medicine and Animal Science,National University of Colombia,Bogotá,Colombia |
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Abstract: | Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety
of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization)
BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from
the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became
characteristic after 36–48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference
strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves.
These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher
burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot
on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just
one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful
as seeds to develop new monovalent vaccines. |
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Keywords: | Bovine herpesvirus 1 Genital infection Respiratory infection Latent infection |
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