Acinetobacter baumannii outer membrane protein A modulates the biogenesis of outer membrane vesicles |
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Authors: | Moon Dong Chan Choi Chul Hee Lee Jung Hwa Choi Chi-Won Kim Hye-Yeon Park Jeong Soon Kim Seung Il Lee Je Chul |
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Institution: | (1) Department of Microbiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea;(2) Division of Life Science, Korea Basic Science Institute, Daejeon, 305-806, Republic of Korea;(3) Division of Magnetic Resonance Research, Korea Basic Science Institute, Chungbuk, 363-883, Republic of Korea;(4) Present address: Department of Periodontology, University of Florida, Gainesville, Florida, USA; |
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Abstract: | Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of peptidoglycan.
This study investigated the role of AbOmpA in the biogenesis of A. baumannii OMVs. Quantitative and qualitative approaches were used to analyze OMV biogenesis in A. baumannii ATCC 19606T and an isogenic ΔAbOmpA mutant. OMV production was significantly increased in the ΔAbOmpA mutant compared to
wild-type bacteria as demonstrated by quantitation of proteins and lipopolysaccharides (LPS) packaged in OMVs. LPS profiles
prepared from OMVs from wild-type bacteria and the ΔAbOmpA mutant had identical patterns, but proteomic analysis showed different
protein constituents in OMVs from wild-type bacteria compared to the ΔAbOmpA mutant. In conclusion, AbOmpA influences OMV
biogenesis by controlling OMV production and protein composition. |
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