Improved assessment of viability and germination of Cattleya (Orchidaceae) seeds following storage |
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Authors: | Silvério Takao Hosomi Ceci Castilho Custódio Philip T Seaton Timothy R Marks Nelson Barbosa Machado-Neto |
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Institution: | (1) Biology Department, UNOESTE, Rua Jos? Bongiovani, 700, SP, Presidente Prudente, 1905-680, Brazil;(2) Agronomy Department, UNOESTE, Rod Raposo Tavares, km 572, SP, Presidente Prudente, 19067-175, Brazil;(3) Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex, RH17 6TN, UK; |
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Abstract: | Effective seed storage methods for orchid species provide a unique opportunity to preserve large amounts of germplasm. Early
evaluation of the physiological status of seeds is useful for selecting seed lots for conservation. To score seed quality,
seeds from nine Cattleya species (Cattleya granulosa, Cattleya hegeriana, Cattleya intermedia, Cattleya mossiae, Cattleya purpurata, Cattleya sanguiloba, Cattleya tenuis, Cattleya tigrina and Cattleya walkeriana) were germinated on Knudson C medium and viability was measured using a tetrazolium (TZ) test following pre-conditioning
in a 10% sucrose solution, both before and after storage at −18°C for 3 mo. Tetrazolium test results were quantified using
digital imaging of germinating seeds on a flatbed scanner. Seed viability (with or without pre-conditioning in sucrose), germination,
Germination Velocity Index (GVI) and the correlation between viability measurements and germination were also evaluated. Both
the tetrazolium test and the germination results showed that there was no loss of viability after storage, although in five
seed lots, the GVI decreased. Pre-conditioning improved the correlation between viability exhibited in the tetrazolium test
and seed germination. Image analysis proved to be a powerful tool for analysing these small seeds. |
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