Genome-wide DNA methylation analysis in precursor B-cells |
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Authors: | Md Almamun Benjamin T Levinson Susan T Gater Robert D Schnabel Gerald L Arthur J Wade Davis Kristen H Taylor |
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Affiliation: | 1.Department of Pathology and Anatomical Sciences; University of Missouri-Columbia; Columbia, MO USA;2.Division of Research; Texas A&M University; College Station, TX USA;3.Division of Animal Sciences; University of Missouri-Columbia; Columbia, MO USA;4.Management and Informatics; University of Missouri-Columbia; Columbia, MO USA;5.Department of Statistics; University of Missouri-Columbia; Columbia, MO USA |
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Abstract: | DNA methylation is responsible for regulating gene expression and cellular differentiation and for maintaining genomic stability during normal human development. Furthermore, it plays a significant role in the regulation of hematopoiesis. In order to elucidate the influence of DNA methylation during B-cell development, genome-wide DNA methylation status of pro-B, pre-BI, pre-BII, and naïve-B-cells isolated from human umbilical cord blood was determined using the methylated CpG island recovery assay followed by next generation sequencing. On average, 182–200 million sequences were generated for each precursor B-cell subset in 10 biological replicates. An overall decrease in methylation was observed during the transition from pro-B to pre-BI, whereas no differential methylation was observed in the pre-BI to pre-BII transition or in the pre-BII to naïve B-cell transition. Most of the methylated regions were located within intergenic and intronic regions not present in a CpG island context. Putative novel enhancers were identified in these regions that were differentially methylated between pro-B and pre-BI cells. The genome-wide methylation profiles are publically available and may be used to gain a better understanding of the involvement of atypical DNA methylation in the pathogenesis of malignancies associated with precursor B-cells. |
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Keywords: | DNA methylation enhancer next-generation sequencing precursor B-cell umbilical cord blood |
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