AgNO3 boosted high-frequency shoot regeneration in Vigna mungo (L.) Hepper |
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| Authors: | Muruganantham Mookkan Ganapathi Andy |
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| Institution: | 1.Department of Biotechnology and Genetic Engineering; School of Biotechnology; Bharathidasan University; Tiruchirappalli, Tamil Nadu, India;2.The S. Tolkowsky Laboratory; Department of Plant Pathology-The Virology Unit; Agricultural Research Organization; Volcani Center; Bet Dagan, Israel;3.Department of Horticulture; University of Georgia Tifton Campus; Tifton, GA USA;4.Division of Plant Sciences; University of Missouri; Columbia, MO USA |
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| Abstract: | In order to further increase shoot regeneration frequency of Vigna mungo (L.) Hepper., the effects of AgNO3 on this process was investigated in this study. The shoot tip and cotyledonary node explants were cultured on MS salts B5 Vitamins medium containing BA+TDZ+Ads+AgNO3 for multiple shoot induction. AgNO3 influenced the shoot bud formation and their subsequent proliferation. The best medium composition for multiple shoot induction was BA, TDZ combination with Ads and AgNO3 in MSB5 medium. Maximum 39 shoots in cotyledonary node and 22 shoots in shoot tip were obtained per explants after 4 – 6 wk. of culture. Elongation and rooting were performed in GA3 (0.6mg/l) and IBA (0.4mg/L) containing media respectively. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 78%. |
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| Keywords: | black gram direct shoot regeneration thidiazuron silver nitrate subculture |
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