Overexpression of archaeal proteins in Escherichia coli |
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| Authors: | Rosalind Kim Steve J. Sandler Stanley Goldman Hisao Yokota Alvin J. Clark Sung-Hou Kim |
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| Affiliation: | (1) Structural Biology Department, Physical Biosciences Division of Lawrence Berkeley National Laboratory and Department of Chemistry, University of California Berkeley, USA;(2) Molecular and Cell Biology, University of California Berkeley, USA |
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| Abstract: | Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare tRNAs (tRNA arg(de)AGA/AGG and tRNA ile(de)AUA. © Rapid Science Ltd. 1998 |
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