Lack of association between human erythrocyte membrane acetylcholinesterase and osmotic fragility. |
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| Authors: | S S Kaplay |
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| Affiliation: | 1. LIEN EA 4685, Laboratoire de Pharmacie industrielle et de Cosmétologie, Nantes Université, Faculté de Pharmacie, 44000 Nantes, France;2. Service de Psychiatrie, Unité Espace, CHU de Nantes, 85, rue Saint-Jacques, 44000 Nantes, France;3. Laboratoire Rivadis, 9, rue Marcel-Sembat, 44100 Nantes, France;1. Department of Pharmacy and Pharmaceutical Sciences, National University of Singapore, 18 Science Drive 4, 117543, Singapore;2. Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, MD6 Centre for Translational Medicine, 14 Medical Drive, Singapore, 117599, Singapore;3. Institute for Health Innovation and Technology (iHealthtech), National University of Singapore, E7, 15 Kent Ridge Crescent, Singapore, 119276, Singapore;4. Division of Gastroenterology & Hepatology, Department of Medicine, National University Hospital, Singapore;1. Department for Experimental Toxicology and Pharmacology, National Poison Control Centre, Military Medical Academy, Crnotravska 17, 11000, Belgrade, Serbia;2. Medical Faculty of the Military Medical Academy, University of Defence, Crnotravska 17, 11000, Belgrade, Serbia;3. Department of Chemistry, Faculty of Science, University of Hradec Kralove, Rokitanskeho 62, 500 03, Hradec Kralove, Czech Republic;4. University of Belgrade – Faculty of Chemistry, Department of Analytical Chemistry Studenski trg 16, 11000, Belgrade, Serbia;5. University of Belgrade – Institute for Medical Research, National Institute of the Republic of Serbia, Department for Cardiovascular Research, Dr Subotića 4, 11 000, Belgrade, Serbia;6. Special Police Unit, Ministry of Interior, Trebevićka 12/A, 11 030, Belgrade, Serbia;7. Centre for Biomedical Research, Faculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000, Banja Luka, Bosnia and Herzegovina;8. Department of Human Genetics, Faculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000, Banja Luka, Bosnia and Herzegovina;9. Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000, Banja Luka, Bosnia and Herzegovina;10. University of Belgrade – Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Vojvode Stepe 450, 11000, Belgrade, Serbia;11. Veterinary Services Center, Military Health Department, Crnotravska 17, 11000, Belgrade, Serbia;12. Biomedical Research Center, University Hospital Hradec Kralove, 50005, Hradec Kralove, Czech Republic |
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| Abstract: | It has been postulated that acetylcholinesterase has a role in the maintenance of fragility and permeability properties of erythrocyte membrane. Osmotic fragility of erythrocyte membrane, acetylcholinesterase activity, and its apparent Km values were determined in the whole cell suspension and membrane preparations (± 0.5% Triton X-100) obtained from children suffering from severe protein-calorie deficiency both before and after dietary treatment. Osmotic fragility of the erythrocyte membrane was decreased at the time of admission and became normal after nutritional therapy. However, under none of the assay conditions were there differences either in the activity or in the apparent Km (acetylthiocholine) of acetylcholinesterase which were associated with the change in osmotic fragility properties of the membrane. |
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