Modulation of MCP-1 and iNOS by 50-Hz sinusoidal electromagnetic field. |
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Authors: | Marcella Reale Maria Anna De Lutiis Antonia Patruno Lorenza Speranza Mario Felaco Alfredo Grilli Maria Antonietta Macrì Silvia Comani Pio Conti Silvano Di Luzio |
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Institution: | Department of Oncology and Neuroscience, Universita degli Studi G.D'Annunzio, Chieti, Italy. mreale@unich.it |
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Abstract: | The purpose of this study was to investigate whether overnight exposure to 1 mT-50 Hz extremely low-frequency sinusoidal electromagnetic field (EMF) affects the expression and production of inducible nitric oxide synthase (iNOS) and monocyte chemotactic protein-1 (MCP-1) in human monocytes. RT-PCR and Western blot analysis demonstrate that EMF exposure affects the expression of iNOS and MCP-1 in cultured human mononuclear cells at the mRNA level and protein synthesis. Interestingly, the effects of EMF exposure clearly differed with respect to the potentiation and inhibition of iNOS and MCP-1 expression. Whereas iNOS was down-regulated both at the mRNA level and at the protein level, MCP-1 was up-regulated. These results provide helpful information regarding the EMF-mediated modulation of the inflammatory response in vivo. However, additional studies are necessary to demonstrate that EMF acts as a nonpharmacological inhibitor of NO and inducer of MCP-1 in some diseases where the balance of MCP-1 and NO may be important. |
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