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The Structure of the Oocyst and the Excystation Process of Isospora marquardti sp. n. from the Colorado pika, Ochotona princeps
Authors:DONALD W. DUSZYNSKI  JOHN T. BRUNSON
Affiliation:Dept. of Biology, Univ. of New Mexico, Albuquerque, New Mexico 87106 and Dept. of Zoology, State Univ. College, Oswego, New York 13126
Abstract:SYNOPSIS. Oocysts of Isospora marquardti sp. n. from the Colorado pika, Ochotona princeps , have spheroid oocysts, 30.5 (23–36) μm in diameter, and ovoid sporocysts, measuring 19.3 × 12.0 (17–22 × 10–14) μm. A polar body, 2 × 4 μm, a spheroid sporocyst residuum (8.3 μm in diameter), a Stieda body, and a distinct substiedal body (3 × 3 μm) are present. A micropyle and an oocyst residuum are absent. Excysted sporozoites, averaging 3.0 × 18.5 (2–4 × 15–20) μm, contain 2 refractile globules, 1 on each side of the nucleus with a prominent nucleolus.
The sporozoite excystation process using a trypsin-sodium taurocholate fluid is described.
Keywords:Isospora    marquardti sp. n.    Colorado pika (Ochotona princeps) from    sporulated oocysts    structure of    taxonomy
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