Tissue distribution of P2X4 receptors studied with an ectodomain antibody |
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| Authors: | Xuenong Bo Stefania L. Nori Ralf Schoepfer Geoffrey Burnstock R. Alan North |
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| Affiliation: | (1) Institute of Molecular Physiology, University of Sheffield, Sheffield, S10 2TN, UK;(2) Department of Anatomy and Cell Biology, Catholic University, 00168 Rome, Italy;(3) Wellcome Laboratory for Molecular Pharmacology, University College London, London, WC1E 6BT, UK;(4) Autonomic Neuroscience Institute, Royal Free and University College Medical School, London, NW3 2PF, UK;(5) Present address: Academic Department of Neurosurgery, Barts and The London School of Medicine and Dentistry, Medical Science Building, Queen Mary, University of London, Mile End Road, London E1 4NS, UK |
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| Abstract: | A monoclonal antibody was developed to the extracellular domain of the rat P2X4 receptor. The antibody was highly selective among all rat P2X receptor subunits, and recognised only the oligomeric, non-denatured form of the P2X4 receptor. Immunohistochemistry showed an extensive pattern of distribution throughout the central and peripheral nervous systems, the epithelia of ducted glands and airways, smooth muscle of bladder, gastrointestinal tract, uterus, and arteries, uterine endometrium and fat cells. The protein was identified by Western blotting in membrane extracts of these tissues, and the ectodomain antibody immunoprecipitated a protein that was recognised with a P2X4 receptor C terminus antibody. The findings indicate that the P2X4 receptor subunit has a very extensive distribution among mammalian tissues, and this suggests possible new functional roles.The work was supported by the Wellcome Trust (R.S., R.A.N.) and the British Heart Foundation (G.B.) |
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| Keywords: | P2X4 receptor Purinoceptor ATP Immunoprecipitation Immunohistochemistry Rat (Wistar) |
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