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Effect of chitosan on UASB treating POME during a transition from mesophilic to thermophilic conditions |
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| Authors: | Khemkhao Maneerat Nuntakumjorn Boonyarit Techkarnjanaruk Somkiet Phalakornkule Chantaraporn |
| Affiliation: | a The Joint Graduate School of Energy and Environment, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand b Department of Chemical Engineering, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand c National Center for Genetic Engineering and Biotechnology (BIOTEC), Bangkok 10150, Thailand d Department of Chemical Engineering, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand e Research and Technology Center for Renewable Products and Energy, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, Thailand |
| Abstract: | The effects of chitosan addition on treatment of palm oil mill effluent were investigated using two lab-scale upflow anaerobic sludge bed (UASB) reactors: (1) with chitosan addition at the dosage of 2 mg chitosan per g volatile suspended solids on the first day of the operation (R1), (2) without chitosan addition (the control, R2). The reactors were inoculated with mesophilic anaerobic sludge which was acclimatized to a thermophilic condition with a stepwise temperature increase of 5 °C from 37 to 57 °C. The OLR ranged from 2.23 to 9.47 kg COD m−3 day−1. The difference in biogas production rate increased from non-significant to 18% different. The effluent volatile suspended solids of R1 was 65 mg l−1 lower than that of R2 on Day 123. 16S rRNA targeted denaturing gradient gel electrophoresis (DGGE) fingerprints of microbial community indicated that some methanogens in the genus Methanosaeta can be detected in R1 but not in R2. |
| Keywords: | Chitosan UASB Thermophilic Methanogen archaea 16S rRNA gene |
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