Comparison of different procedures of DNA analysis for sex identification in the endangered bearded vulture (<Emphasis Type="Italic">Gypaetus barbatus</Emphasis>) |
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| Authors: | Cristina Belén García Jesús Antonio Insausti Juan Antonio Gil Ángel de Frutos Manuel Alcántara Javier González María Rebeca Cortés José Ignacio Bonafonte María Victoria Arruga |
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| Institution: | (1) Laboratory of Cytogenetics and Molecular Genetics, Faculty of Veterinary Science, University of Zaragoza, C/Miguel Servet, 177, 50013 Zaragoza, Spain;(2) Centro de Recuperación de Fauna Silvestre La Alfranca, Zaragoza, Spain;(3) Fundación para la Conservación del Quebrantahuesos, Zaragoza, Spain;(4) Departamento de Medio Ambiente, Servicio de Biodiversidad, Gobierno de Aragón, Zaragoza, Spain;(5) Surgery Unit, Faculty of Veterinary Science, University of Zaragoza, Zaragoza, Spain |
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| Abstract: | During the last century, bearded vulture populations have declined and are threatened by extinction in Europe. Conservation
efforts such as captive-bird breeding programs require the knowledge of the sex of individuals. The bearded vulture is difficult
to sex morphologically because it is sexually monomorphic. Until now, there were no published genetic methods to sex this
species. In our study, we tested different methods based on polymerase chain reaction analysis of the chromobox-helicase-DNA
binding protein gene. This gene is located on both sex chromosomes, but the two copies differ in size depending on chromosomal
location. Differences can be detected by digestion with restriction enzymes or with the amplification refractory mutation
system technique. These methods are quick, accurate, and inexpensive and allow a large scale sex typing of bearded vultures. |
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| Keywords: | Gypaetus barbatus Sexing CHD gene ARMS Conservation program |
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