Increased cellulose hydrolysis by Bacteroides cellulosolvens in a simplified synthetic medium |
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Authors: | William D Murray |
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Institution: | Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada K1A 0R6 |
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Abstract: | The nutrient requirements of the cellulolytic anaerobe Bacteroides cellulosolvens were determined, and a new synthetic medium was formulated for its growth. B. cellulosolvens showed optimum cellobiose consumption and product formation in medium containing 40 mM ammonia nitrogen, 3 mM phosphate, 1 mM sulfide, 100 μM magnesium and 45 μM iron. This microbe had an essential-vitamin requirement for biotin; while zinc, manganese and copper slightly stimulated cellobiose degradation. In the new synthetic medium B. cellulosolvens was able to degrade 30% more cellulose. |
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Keywords: | cellulose hydrolysis synthetic medium nutrition |
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