Studies on cell-free metabolism: Ethanol production by extracts of Zymomonas mobilis

Using a cell-free extract of Zymomonas mobilis, it has been possible to achieve rapid and sustained ethanol production from added glucose. In one example 18% glucose was totally converted to 9% (w/v) ethanol. The controls on the glycolytic enzymes have been investigated by measuring metabolite levels during the experiment. No substantial accumulations of intermediates occurred when ATP production by the glycolytic metabolism was correctly balanced by ATPase activity. But as alcohol levels increased, some inhibitions of glucose 6-phosphate and pyruvate removal became apparent.