Efficient expression of a promoter-controlled gene: Tandem promoters of lambda PR and PL functional in enteric bacteria |
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Authors: | Yoshikatsu Murooka Isao Mitani |
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Affiliation: | Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Saijo, Higashi-Hiroshima 724, Japan |
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Abstract: | We describe an expression vector that functions in enteric bacteria. The vector contains the coliphage λ promoters PR and PL and entire PR and PL operators in tandem upstream from the multiple cloning sites containing the kanamycin-resistant gene. The vector also specifies a ribosome binding site and a thermolabile repressor, cI857, and the PRM promoter. These promoters as well as lacUV5 and trp promoters were inserted into the EcoRI site of pKO-1 plasmid so that they drove the expression of a reporter gene, galactokinase (galK). The PRPL promoter showed the highest efficiency of galK expression in the Escherichia coli strain K12ΔH1Δtrp; it was strong in Klebsiella aerogenes, and weak in Serratia marcescens and Citrobacter freundii. |
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Keywords: | expression vector tandem promoter galactokinase enteric bacteria |
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