Comprehensive proteome analysis in Cenococcum geophilum Fr. as a tool to discover drought-related proteins |
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| Authors: | Kerner René Delgado-Eckert Edgar Del Castillo Estela Müller-Starck Gerhard Peter Martina Kuster Bernhard Tisserant Emilie Pritsch Karin |
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| Affiliation: | 1. Section of Forest Genetics, Technische Universität München, Hans-Carl-von-Carlowitz-Platz 2, D-85354 Freising, Germany;2. Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany;3. Department of Biosystems Science and Engineering, Computational Biology Group, ETH Zürich, Mattenstrasse 26, CH-4058 Basel, Switzerland;4. Chair of Proteomics and Bioanalytics, Technische Universität München, Emil-Erlenmeyer-Forum 5, D-85354 Freising, Germany;5. Swiss Federal Institute for Forest, Snow and Landscape Research WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland;6. Center for Integrated Protein Science Munich, Munich, Germany;g Institute of Soil Ecology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany;h UMR 1136 INRA / Nancy Université, Interactions Arbres / Micro-organismes, Centre de Nancy, 54280 Champenoux, France |
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| Abstract: | Cenococcum geophilum is a widely distributed ectomycorrhizal fungus potentially playing a significant role in resistance and resilience mechanisms of its tree hosts exposed to drought stress. In this study, we performed a large scale protein analysis in pure cultures of C. geophilum in order to gain first global insights into the proteome assembly of this fungus. Using 1-D gel electrophoresis coupled with ESI-MS/MS, we indentified 638 unique proteins. Most of these proteins were related to the metabolic and cellular processes, and the transport machinery of cells. In a second step, we examined the influence of water deprivation on the proteome of C. geophilum pure cultures at three time points of gradually imposed drought. The results indicated that 12 proteins were differentially abundant in mycelia subjected to drought compared to controls. The induced responses in C. geophilum point towards regulation of osmotic stress, maintainance of cell integrity, and counteracting increased levels of reactive oxygen species formed during water deprivation. |
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| Keywords: | ALDH, aldehyde dehydrogenase BSA, bovine serum albumin DNPH, dinitrophenylhydrazine FA, formic acid FDR, false discovery rate GO, gene ontology LEA protein, late embryogenesis abundant protein Mgf, mascot generic file NAC, nascent polypeptide associated complex NSAF, normalized spectral abundance factor PC, polycarbonate PK, pyruvate kinase. |
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