Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA |
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Authors: | Rodriguez David Longo Ana V Zamudio Kelly R |
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Affiliation: | Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, USA. dr376@cornell.edu |
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Abstract: | We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications. |
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