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Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA
Authors:Rodriguez David  Longo Ana V  Zamudio Kelly R
Affiliation:Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, USA. dr376@cornell.edu
Abstract:We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.
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