Rat epiphyseal cells in culture: Responsiveness to bone-seeking hormones |
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| Authors: | J. Levy Z. Shimshoni D. Somjen E. Berger N. Fine M. Silbermann I. Binderman |
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| Affiliation: | (1) Department of Orthodontics, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel;(2) Hard Tissues Unit, Ichilov Hospital, Tel Aviv, Israel;(3) The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel;(4) Rappaport Family Institute for Research in the Medical Sciences, Division of Morphological Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel |
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| Abstract: | Summary Cell cultures derived from young rat epiphyseal cartilage were grown for approximately 2 wk in BGJ b medium supplemented with 10% fetal bovine serum to reach confluence. These cells were identified as chondrocytes as checked by morphology, the presence of alkaline phosphatase, and a positive type II collagen antibody reaction. The cells also responded to different hormonal treatment. Parathyroid hormone (PTH) increased cyclic AMP production by 50% within 15 min of treatment, whereas prostaglandin E2 (PGE2) caused an increase of 160%. Calcitonin (CT) did not affect cAMP production in these cells. DNA synthesis 24 h after hormonal treatment was increased by PTH (2.5-fold) and PGE2 (2-fold), but not by CT. Among the vitamin D metabolites, 24,25(OH)2D3 increased significantly the [3H]thymidine incorporation into DNA, whereas 1,25(OH)2D3 effect was minimal. These results provide evidence for the use of these cell cultures as a model for cartilage in vitro when studying biological and hormonal responsiveness. |
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| Keywords: | epiphyseal cartilage cells parathyroid hormone prostaglandin E2 calcitonin cAMP DNA vitamin D metabolites |
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