金花茶胚状体中游离氨基酸的含量及花氨酸、丝氨酸对胚状体发育的影响

本文报道金花茶Camellia chrysantha(HU)Tuyama胚状体培养中,具有正常产生次级胚能力的材料(第一类)和产生次胚能力差的材料(第二类)的游离氨基酸测定结果,以及脯氨酸、丝氨酸对胚状体发生的培养结果。所检出的氨基酸含量,第一类材料为第二类材料的1—5倍;且第二类材料中未能检出脯氨酸,苯丙氨和酪氨酸。在此基础上培养发现:脯氨酸和低浓度(50mmol、100mmol)的丝氨酸促进胚状体发生与鲜重增加,脯氨酸、丝氨酸和苯丙氨酸以50mmol混合使用效果最好,而且除色氨酸外,这些外加氨基酸对胚体形成频率、数目和鲜重的影响之间都有平行关系。

THE CONTENT OF FREE AMINO ACIDS IN SOMATIC EMBRYOIDS OF COMELLIA CHRYSANTHA (HU) TUYAMA AND EFFECTS OF PRO, SER ON SOMATIC EMBRYOGENESIS

This paper reports the free amino acids content of two culture materials in somatic embryogenesis of Comellia chrysantha (HU) Tuyama, one of which has the normal ability of producing secondary embryo(The first), and the other has the poorer ability(The second). Amino acids in the first material is one to five-fold that of in the second, and no Phe, Pro and Tyr were detected in the second material.Based on-mationed results, we utilized Pro, Ser, Phe, Trp and mixed AA to s matic embryogenesis culture. The experiment shows that Pro and low con(?)entration(50mM, 100mM)Ser promote the somatic embryogenesis and fresh weight in(?)rease, mixed 50mM Pro, Ser and Phe have the best effect, all of these additional AA except Tyr has a parallel relationship among frequence of embryogenesis, amounts and fresh weight.