Simultaneous production of anthocyanin and triterpenoids in suspension cultures of Perilla frutescens |
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Authors: | J W Wang Z H Xia J H Chu R X Tan |
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Institution: | Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China |
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Abstract: | When cultivated in Murashige & Skoog medium supplemented with 0.2 mg l?1 2,4-dichlorophenoxy acetic acid and 0.5 mg l?1 6-benzyladenine, Perilla frutescens cells in suspension culture grew rapidly reaching about 13.6 g dry wt l?1 after 12 days. The cell line produced both anthocyanin 0.9 g l?1 and triterpenoids: 16 mg l?1 oleanolic acid (OA), 25 mg l?1 ursolic acid (UA) and 14 mg l?1 tormentic acid (TA). When P. frutescens cells of 7-day-old cultures were exposed to a yeast elicitor at 0.5–5% (v/v) for 7 days, it was found that anthocyanin content peaked at 10.2% of dry weight with yeast elicitor at 1% (v/v) whereas the maximum production of oleanolic acid and ursolic acid in cultures treated with 2% (v/v) yeast elicitor was 19 and 27 mg l?1, a 46 and 24% increase over the control, respectively. This is the first report of simultaneous production of both anthocyanin and triterpenoids in a single culture system. |
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Keywords: | Cell suspension culture Elicitation Perilla frutescens Labiatae Anthocyanin Triterpenoids |
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