Sister chromatid exchanges in isolabeling |
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Authors: | D Ann Gibson D M Prescott |
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Institution: | Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colo 80302, USA |
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Abstract: | Isolabeling observed by autoradiography in sister chromatids at the second or later metaphases after incorporation of 3H-thymidine has sometimes been ascribed to an exchange between the multiple DNA duplexes in polynemic sister chromatids. An analysis reported here on the frequency and size of isolabeled regions in chromosomes of the rat kangaroo shows that all isolabeling can be accounted for by sister chromatid exchanges coupled with the image spread that can occur in tritium autoradiographs. Hence, in this case it becomes unnecessary to postulate binemy or polynemy to explain isolabeling. |
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