Purification, Characterization, and Antifungal Activity of Chitinase from Streptomyces venezuelae P10 |
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Authors: | G. Mukherjee S. K. Sen |
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Affiliation: | (1) Division of Microbiology, School of Life Sciences, Visva-Bharati (Central University), Santiniketan, 731235, India |
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Abstract: | Streptomyces venezuelae P10 could produce extracellular chitinase in a medium containing 0.6% colloidal chitin that was fermented for 96 hours at 30°C. The enzyme was purified to apparent homogeneity with 80% saturation of ammonium sulfate as shown by chitin affinity chromatography and DEAE-cellulose anion-exchange chromatography. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 66 kDa. The chitinase was characterized, and antifungal activity was observed against phytopathogens. Also, the first 15 N-terminal amino-acid residues of the chitinase were determined. The chitin hydrolysed products were N-acetylglucosamine and N, N’-diacetylchitobiose. |
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