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The use of real-time PCR for quantitative determination of some propionic bacteria inhabiting the human skin |
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| Authors: | A G Globa Ya I Alekseev V G Arzumanyan V A Zaborova A A Guridov |
| Institution: | 1. Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, ul. Timiryazevskaya 42, Moscow, 127550, Russia 2. Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia 3. Sechenov Moscow State Medical University, Moscow, Russia |
| Abstract: | A test system has been developed for determination of propionic bacterial species inhabiting the human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum, and P. avidum. This system was used for analysis of wash samples from the skin of 17 pentathlon sportsmen and 16 students. All three species of propionic bacteria were found in all skin wash samples. However, contamination with P. acnes was two times higher in the control group than in the group of pentathlon sportsmen. |
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