Homology modeling and assigned functional annotation of an uncharacterized antitoxin protein from Streptomyces xinghaiensis |
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| Authors: | Arafat Rahman Oany Md Shahabuddin Ahmed Nasreen Jahan Md Abdul Latif Shahin Mahmud Md. Ahmed Hossain Fatema Akter Hasibul Haque Rakib Md. Shariful Islam |
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| Affiliation: | 1.Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Santosh, Tangail-1902, Bangladesh;2.Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh |
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| Abstract: | Streptomyces xinghaiensis is a Gram-positive, aerobic and non-motile bacterium. The bacterial genome is known. Therefore, it is ofinterest to study the uncharacterized proteins in the genome. An uncharacterized protein (gi|518540893|86 residues) in thegenome was selected for a comprehensive computational sequence-structure-function analysis using available data and tools. Subcellularlocalization of the targeted protein with conserved residues and assigned secondary structures is documented. Sequencehomology search against the protein data bank (PDB) and non-redundant GenBank proteins using BLASTp showed differenthomologous proteins with known antitoxin function. A homology model of the target protein was developed using a knowntemplate (PDB ID: 3CTO:A) with 62% sequence similarity in HHpred after assessment using programs PROCHECK and QMEAN6.The predicted active site using CASTp is analyzed for assigned anti-toxin function. This information finds specific utility inannotating the said uncharacterized protein in the bacterial genome. |
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| Keywords: | antitoxin homology modeling active-site residues prediction hypothetical protein Streptomyces xinghaiensis |
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