Evolution of satellite DNA sequences in two tribes of Bovidae: A
cautionary tale |
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| Authors: | Mariella Nieddu Roberto Mezzanotte Giuseppina Pichiri Pier Paolo Coni Gian Luca Dedola Maria Luisa Dettori Michele Pazzola Giuseppe Massimo Vacca Renato Robledo |
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| Affiliation: | 1.Department of Biomedical Sciences, University of Cagliari, Monserrato, Italy;2.Department of Surgical Sciences, University of Cagliari, Cagliari, Italy;3.Department of Veterinary Medicine, University of Sassari, Sassari, Italy |
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| Abstract: | Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalismusimon, were used as probes for hybridization on genomic DNA and onmetaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 aresequences respectively belonging to the 1.715 and 1.714 DNA satellite I families.Southern blots and fluorescence in situ hybridization experimentsshowed completely coherent results: the Bovini probe Bt1 hybridized only to membersof the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1hybridized only to members of the Caprini tribe and not to members of Bovini.Hybridization signals were detected in the heterochromatic regions of everyacrocentric autosome, except for two pairs of autosomes from Caprahircus that did not show hybridization to probe Om1. No signal wasdetected on X and Y chromosomes or on bi-armed autosomes. Remarkably, probe Om1showed almost 100% homology with a bacterial sequence reported inHelicobacter pylori. |
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| Keywords: | Bovidae satellite DNA fluorescence in situ hybridization pericentromeric region |
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