Preliminary Study on Linkage Mapping Based on Microsatellite DNA and AFLP Markers Using Homozygous Clonal Fish in Ayu (<Emphasis Type="Italic">Plecoglossus altivelis</Emphasis>)期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Preliminary Study on Linkage Mapping Based on Microsatellite DNA and AFLP Markers Using Homozygous Clonal Fish in Ayu (Plecoglossus altivelis)

Authors:	Tomohisa?Watanabe,Hironobu?Fujita,Kimio?Yamasaki,Shingo?Seki,Nobuhiko?Taniguchi author-information" > author-information__contact u-icon-before" > mailto:nobuhiko@bios.tohoku.ac.jp" title=" nobuhiko@bios.tohoku.ac.jp" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Sendai, Miyagi 981-8555, Japan;(2) Department of Aquaculture, Faculty of Agriculture, Kochi University, B-200, Monobe, Nankoku, Kochi 783-8502, Japan;(3) Wakayama Prefectural Freshwater Fisheries Center, Chikazuki 32-3, Momoyama, Wakayama 649-6112, Japan

Abstract:	A mapping referential family (F₁) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.

Keywords:	AFLPs ayu (Plecoglossus altivelis) linkage map microsatellite DNA.
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